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extracellular LDH) was used as an index of necrotic cell death and LDH present in the adherent viable cells as intracellular LDH (LDHi)

It is unclear if this is due to the limited number of patients examined at a molecular level, or to the fact that variations in the extracellular domain have limited functional significance, or perhaps because the phenotype produced by extracellular mutations differs from the classic phenotype, with patients escaping ascertainment

Second, mechanistic insights were mainly derived from transcriptomic analysis and limited protein validation, without pathway-specific functional interference to establish causality

Floh et al., 1997)