After their nucleotide sequences were confirmed, the coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were cut out as Kpn I- Xho I fragments and were inserted between the Kpn I and Xho I sites in the yeast expression vector pYES2 (Life Technologies)
In some embodiments, the amount of thickening agent (e.g., a gelling agent) in any formulation described herein is about 0.01%, about 0.02%, about 0.03%, about 0.04%, about 0.05%, about 0.06%, about 0.07%, about 0.08%, about 0.09%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 5%, about 10%, or about 15% of the total weight of the formulation
doi: 10.1186/1471-2407-13-59 143 ProtopsaltisNJLiangWNudlemanEFerraraN
Caffeic acid phenethyl ester protects bloodbrain barrier integrity and reduces contusion volume in rodent models of traumatic brain injury
Therefore, K63-linked ubiquitination contributes to SHMT1 stabilization