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ODN 1826 (Type B) Endotoxin-free (sterile) Pack Size:1 mg The cell lysis buffers used

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Description

The cell lysis buffers used are much stronger than RIPA buffers

Obtaining quality cell suspension from fresh and/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification

PRKD1 (PKCµ)

The procedure can be completed in less than 45 minutes with a final yield of 10-50 µg protein/sample

Protein extraction with high concentration denaturants is relatively effective

ODN 1826 (Type B) Endotoxin-free (sterile) Pack Size:1 mg The cell lysis buffers usedUnmethylated CG dinucleotides within particular sequence contexts are responsible for the immunostimulatory activity of bacterial DNA. Synthetic oligonucleotides (ODN) that contain such CpG motifs (CpG ODNs) mimic microbial DNA. The innate immune system of vertebrates has the ability to recognize CpG motifs in microbial DNA via the Toll like receptor (TLR) 9 if the CpG ODN were free of additional immune stimulatory contaminants often present in

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