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For two-step RT-qPCR, cDNA was serially diluted and 2 L was used in a reaction containing a final concentration of 1x DreamTaq PCR mix (Thermo Fisher), 300 nM of forward primer, 300 nM of reverse primer, 100 nM of probe

The ordinance was passed in a 5-1 vote and as of January 1, 2023, cigarette smoking will be officially banned in these areas in the City of Miami Beach

The association began a program this year that urges retailers to require proof of age before selling to anyone and to display signs saying they don't sell to minors

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