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Moreover, the majority of these technologies remain in the preclinical phase, lacking validation in human clinical trials or demonstration of scalable manufacturing processes

Optimization of HPLC-MS/MS conditions All experiments were conducted using an Agilent 1290 Infinity II liquid chromatographic system (Agilent, Palo Alto, CA, USA) equipped with a quaternary high-pressure pump, a thermostated autosampler, a thermostated column compartment, and a diode array UV detector

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Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturers instructions, with absorbance measured at 450 nm