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The intestinal microbiota ferments non-digestible food substances called prebiotics

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HPLC measurement of DA, DOPAC and HVA DA and metabolites were separated by injecting 50 l of sample into a Microsorb C18 column (100 4.6 mm column, Analytical Column, Waters) and a mobile phase containing 130 mM NaH 2 PO 4 , 2 mM NaCl, 0.1 mM EDTA, 2 mM OSA (1-octanesulphonic acid sodium) and 12.5% (v/v) methanol, pH 3.71, at a flow rate of 1 ml min 1

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Never freeze reconstituted peptides, as freeze-thaw cycles can damage peptide structure