': : ' = : 2 , 25 / 2 25 = 0,08 = 8
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Reductase enzymatic activity The reductase activities of the WT and mutant (K8A, C20A and K47A) proteins were measured spectrophotometrically by recording the increase in absorbance at 260 nm using a UV-VIS spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) as described previously 37
The experiments were approved by the Institutional Animal Care and Use Committee of The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University (No