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Handling best practices Minimize the time reconstituted vials spend out of refrigeration Never touch the rubber stopper with anything except an alcohol-swabbed needle Draw doses quickly and return vial to refrigerator immediately Use a fresh needle for each vial entry to minimize contamination risk Never share vials between users Contamination is a significant risk with multi-dose vials

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Using bacteriostatic water or adding a sterile preservative like benzyl alcohol (0.9%) can prevent microbial growth for solutions requiring multiple withdrawals

In addition to the lean and ob/ob mice, the study included 3-KO mice and WT controls obtained from genetically verified breeding colonies