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For splice variant G6PD5.5 , the 5 region (N-terminus) was amplified from genomic DNA of Arabidopsis Col-0 wildtype and inserted into the plant expression vector containing G6PD5.4 (already fused to m GFP), thus changing the corresponding part of the N-terminus

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So when you have insulin resistance first, what happens is higher amounts of sugar, then you get higher amounts [00:15:00] of insulin