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'Kowloon Walled City's History'), by Lu Jinzhe, published by Joint Publishing, 1997, ISBN 9789620406829 :

The recommendation is to take between 25100 micrograms per day, as this amount has been shown to maintain healthy levels of vitamin B12 levels in older people

For instance, studies in germ-free or antibiotic-treated mouse and rhesus monkey models have demonstrated reduced levels of BBB tight junction proteins (e.g., claudin-5, occludin) and increased BBB permeability