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Plates were placed in a motorized stage microscope (EVOS Auto FL 2, Thermo Fisher Scientific, Waltham, MA) equipped with an on-stage incubator and maintained at 5% CO 2 and 37C

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5 Mitochondrial ROS imbalance in the molecular pathogenesis of supraspinatus muscle atrophy 5.1 Oxidative stress-induced damage triggered by ROS imbalance 5.1.1 Oxidative modification of proteins, lipids, and DNA Reactive oxygen species (ROS) induce oxidative damage to skeletal muscle macromolecules, disrupting structural integrity and biological function

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