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Furthermore, a key limitation of this study is the absence of in-depth exploration into the molecular mechanisms underlying the niosomal formulations of both treatments (QTLN and QTLN), particularly how they enhance male fertility by modulating specific genetic pathways such as Nrf2/HO-1, sperm DNA damage (include assays such as Sperm Chromatin Dispersion (SCD) test or Comet assay for sperm DNA integrity) as well as the lack of evaluation regarding their potential applicability in humans

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