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Cells were then double-stained with Annexin-V-PE and 7-amino-actinomycin D (7AAD) (PE Annexin V Apoptosis Detection Kit with 7-AAD

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When GABA neurotransmitters bind with the GABAAR on postsynaptic neurons, that leads to the opening of chlorine channels and, finally, the influx of Cl into the cell, which reduces neuronal excitability by hyperpolarization of the membrane (Ghit et al., 2021)

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Additionally, the protein level of PMP70 was higher in the HF/L-LC group than in the HF group ( P = 0023), but no significant difference was found in the HF/H-LC and HF groups ( P = 0574) (Fig