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They competed over the quality of their crop, while plunging deeper into debt to the English merchants in order to display their wealth and maintain a high status among Colonial society

Clonal 143B osteosarcoma cells expressing jAspSnFR3/NucRFP (hereafter referred to as sensor cells) were seeded onto multi-well plates in Dulbeccos modified Eagle medium (DMEM), acclimated for 24 h, and then imaged approximately every 3 h for a total of ~72 h in an Incucyte live-cell imaging system (Methods)

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Cigar Hustler does not list the B-55, but does sell the other sizes