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After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature

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In ALL-derived cell lines and bone marrow mononuclear cells from ALL patients, 7 Wnt antagonists (sFRP1, sFRP2, sFRP4, sFRP5, WIF1, DKK3, and HDPR1) showed abnormal promoter methylation