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have recently shed some light to this problem

The antibody titer was determined as the inverse of the last dilution that was considered positive, with a cut-off value defined as A 450 = 0.20, which was twice as high as that from a pool of normal mice sera (from 30 unimmunized animals)

Limitations The study has a limited number of participants (90 patients), which may restrict the generalizability of the results to the broader population