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For perfusion assays with HeLa cells, NAPstar3b was monitored using the Ex and Em settings as above, HyPer7 was monitored using- Ex: 405 20 nm, Em: 525 50 nm and Ex: 470 40 nm, Em: 525 50 nm

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However, if the solution is cloudy, has a distinct yellow color, or contains any visible particles, its a sign of degradation or contamination and should be discarded immediately

Therefore, they are considered completely safe, even the synthesized versions

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