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It is interesting to note that CP17, which was originally designed to target the lipid-binding pocket within VP1, binds very specifically at a completely separate site and shows no evidence of binding within the targeted pocket

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Why would anyone need Vitamin B12, or want to give themselves B12 shots

Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

doi: 10.3945/jn.108.093237 115