Hi all, 8 days ago i got my first b12 shot (1000 microgram hydroxocobalamin)
gentle warming and extended mixing may be required Do not use organic solvents (acetonitrile, DMSO) unless specifically validated for the peptide Solution is Cloudy or Precipitated Cloudiness may indicate peptide aggregation or poor solubility Try gentle warming to 37 C and slow cooling to room temperature If precipitation persists, the peptide may be degraded or the concentration too high Consider diluting to a lower concentration or using a different buffer (e.g., PBS with 0.1% BSA) Foam Formation Foam is caused by vigorous shaking or forceful injection of water Allow foam to settle naturally (do not shake to disperse) If foam persists, briefly centrifuge the vial at low speed (e.g., 500 g for 2 minutes) To prevent foam: inject water slowly down the vial wall
Whilst true toxicity from too much vitamin B12 is exceptionally rare due to the body's natural excretion mechanisms, elevated B12 levels can occur and may signal underlying health conditions
Therefore, an intramuscular injection of hydroxocobalamin produces higher serum level than the same dose of cyanocobalamin
Reported human side effects are based on anecdote and case reports, not controlled trials