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Additional preclinical work published in Cell Chemical Biology evaluated NNMT inhibition in similar diet-induced obesity models and confirmed the pattern: significant reductions in body weight gain, adipose tissue expansion, and circulating triglycerides compared to untreated controls

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120,253 The helix-turn-helix (HTH) motif that mediates DNA-binding is located at the 3-4 segment that engages successive DNA major grooves, with a degenerate tetrapeptide sequence in 4 that enforces specificity for a particular DNA operator sequence

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