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Cells were cultured in basic Alpha Medium (Corning, New York, USA) with 10% fetal bovine serum (Biowest, Riverside, MO, USA), 20 mM HEPES (Corning, New York, USA) and 1% penicillin-streptomycin (Gibco, Waltham, MA, USA)

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The group is financed with money from a $206 billion settlement that tobacco companies made with Iowa and 45 other states in 1998