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The apparent permeability coefficient ( P app ) was calculated using the following equation: where Q is the change in the amount of CF over the designated period of time ( t ), A is the nominal surface area of the growth supports (i.e., 1.13 and 0.33 cm 2 in case of 3460 and 3470 Transwell inserts, respectively), and C 0 is the initial concentration of CFDA in the donor fluid

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