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All experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals of the Algerian Association for Experimental Science (AASEA) and approved by the Ethics Committee of department of Biological Science of the Jijel University

Among them, glutathione exists in two interconvertible forms: reduced glutathione (GSH) and its oxidized disulfide (GSSG), formed by the linkage of two GSH molecules via a disulfide bond

A comparison of the enzyme-GSH structure reported here with previously described structures reveals rearrangements in a well-defined network of water molecules in the active site