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MerR family members have been the subject of many engineering efforts from different perspectives, facilitated by the fact that these are largely single-site regulators, in which metal specificity is determined, at least part, by the specific nature of the metal binding loop (MBL) (Section 3.2.3)

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After loading, onto a Dionex 0.1 20 mm 5 m C18 nano trap column at a flowrate of 5 L/min, sample was eluted onto an Acclaim PepMap C18 nano column 75 m 50 cm, 2 m 100 at a flow rate of 0.3 L/min