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Stimulation assays for testing the effects of activity on the localization of SVs Surface epitopes of Syt1 were saturated by incubating the neurons in divalent-free buffer (Tyrode buffer without Mg 2+ and Ca 2+ ) with HaloTag-free NbLumSyt1 for 5 min at 37 C

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doi: 10.1371/journal.pone.0254493

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