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ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, United States) connected to a Waverunner LT342 digital oscilloscope (LeCroy, United States) at 30 min ligation time

The supernatant was collected and stored in an Eppendorf tube at 80C until analysis

What researchers should check before each entry A short pre-check keeps bac water handling consistent: Confirm the vial is bacteriostatic water and not preservative-free sterile water

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Journal of Orthopaedic Research , 1999