Cell lysates were pelleted by centrifugation at 30,000 g for 30 min and resuspended in Earles Balanced Salt Solution with Ca 2+ (pH 7.4)
For the LEfSe analysis, an alpha of 0.05 was set for the KruskalWallis tests among classes, and per-sample normalization was performed to bring the sum of the values to 1 M
Li S, Wang Y, Zhang Y, Lu MM, DeMayo FJ, Dekker JD, Tucker PW, Morrisey EE
Scenario 3: Adding 1.5 mL of Bac Water Total Peptide: 30 mg Total Diluent: 1.5 mL Calculation: 30 mg / 1.5 mL = 20 mg per mL Resulting Concentration: This is a highly concentrated solution
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