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The largest numbers of deaths attributable to smoking were in high-income North America with 13 million men and 7 million women, followed by high-income Europe with 15 million and 3 million, respectively

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Screening of functional strains from tobacco After clipping the first-cured tobacco, 10.0 g TL was weighed and inoculated into 250.0 mL flasks containing 90.0 mL sterile water, followed by incubation under 200 rpm shaking at 25 for 60 min

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