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Positive action of high doses of thiamine under metabolic perturbations including surgeries (48, 115, 149, 153155) suggest that cellular protection and/or repair requiring cell differentiation, increase demand of thiamine

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Briefly, the muscle biopsy was first weighed and then cut in ice-cold isolation medium (100 mM sucrose, 100 mM KCl, 50 mM Tris-HCl, 1 mM KH 2 PO 4 , 100 M EGTA, 0.1% BSA