[DOI] [PMC free article] [PubMed] [Google Scholar] 203.Kruger A., Vlok M., Turner S., Venter C., Laubscher G.J., Kell D.B., Pretorius E
After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS
OS refers mainly to an imbalance between oxidation products and the antioxidant system
6 Conclusion and perspectives PCOS, characterized by its complex etiology and heterogeneous clinical manifestations, substantially affects reproductive health and quality of life
Additionally, stress-inducing agents such as tunicamycin, thapsigargin and histidinol have been shown to modulate CHAC1 regulation, indicating its involvement not only during ER stress but also in the context of the ISR ( CHAC1 promoter is the primary mechanism for the transcriptional upregulation of the gene
