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Reagent preparation was as follows: [5,5'-dithio-bis(2-nitrobenzoic acid)] (DTNB) was prepared as a 0.66 mg mL 1 solution in KPE, 40 L of a 250 units mL 1 glutathione reductase (GR) stock was diluted with 3 mL KPE, and -NADPH was also prepared as a 0.66 mg mL 1 solution in KPE

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Meanwhile, its also important to limit your intake of foods that raise cholesterol, such as sugar, processed oils and pre-packaged foods