The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s
Nat Rev Immunol 2:845858 Salaiza-Suazo N, Porcel-Aranibar R, Caneda-Guzman IC, Ruiz-Remigio A, Zamora-Chimal J, Delgado-Dominguez J, Cervantes-Sarabia R, Carrada-Figueroa G, Sanchez-Barragan B, Leal-Ascencio VJ et al (2024) Eosinophils of patients with localized and diffuse cutaneous leishmaniasis: Differential response to Leishmania mexicana, with insights into mechanisms of damage inflicted upon the parasites by eosinophils
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Sances G, Tassorelli C, Pucci E et al (2004) Reliability of the nitroglycerin provocative test in the diagnosis of neurovascular headaches
