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During a wet ball milling process, micronized powder of the polymer is charged in to ball mill containing milling media (e.g., zirconium dioxide beads, silicium nitride beads, polystyrene beads) with an aqueous stabilizer, which is typically a surfactant

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During this process, an artificially designed donor DNA bearing the desirable nucleotide sequence is supplied, which serves as a template to guide the repair of the DSB while incorporating the desirable edits

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[DOI] [PMC free article] [PubMed] [Google Scholar] 47.Nielsen GD, Wolkoff P