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After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h

After that 56-day window, the pen should be discarded even if medication remains inside

All of this is trial data, not a safety guarantee

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