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It is challenging to define cellular senescence in erythrocytes, since these cells are terminally differentiated and their aging is not accompanied by the senescence-associated secretory phenotype (SASP) like in the case of nucleated cells [495]

doi: 10.1093/hmg/ddu542

For longer-term storage, freezing at -20C is recommended to maintain integrity

This is because extreme temperatures and pressure can affect the glass of the vial as well as the plastic stopper