J Biol Chem 283:1447914489
Preparation Condition 2 (EDTA deproteinization 80 o C): Anticoagulants used during collection impacts measures of GSH Suggesting the anticoagulant used during sample collection significantly alters plasma GSH, we found lower levels of free GSH, higher levels of GSSG, and a lower ratio of free GSH to GSSG in the heparinized plasma (Preparation Condition 1) compared to the EDTA-treated plasma (Preparation Condition 2)
Access this article Similar content being viewed by others Abbreviations -GCL: -glutamate cysteine ligase GGT: -glutamyl transferase BSO: L-buthionine-(S, R)-sulfoximine GPx: glutathione peroxidase GSH and GSSG: reduced and oxidized glutathione, respectively MAPK: mitogen-activated protein kinase miR: microRNA Nrf2: NF-E2-related factor 2 ROS: reactive oxygen species REFERENCES Scir, A., Cianfruglia, L., Minnelli, C., Bartolini, D., Torquato, P., Principato, G., Galli, F., and Armeni, T
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