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The effect of CSE treatment on epithelial cells was assessed with the human alveolar basal epithelial cell line A549, the human nasal epithelial cell line RPMI2650, the human bronchial epithelial cell line BEAS-2B, or the mouse lung epithelial cell line MLE12

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Consistent with our findings with ECIG-flavor-2 in the bronchial model, decreased SCGB1A1, increased SLPI, and increased PI3 levels were detected in the sputum from COPD patients compared to smokers without COPD and/or never smokers 78

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