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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB

This results in four alleles of GSTP1 : wild-type GSTP1 *A (Ile105 + Ala114), GSTP1 *B (Val105 + Ala114), GSTP1 *C (Val105 + Val114), and GSTP1 *D (Ile105 + Val114)