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and was read on a MAGPIX plate reader with the xPONENT software (Luminex)

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was amplified as an internal control gene with the following primers: 5-CGG AGT CAA CGG ATT TGG TC-3 (forward) and 5-AGC CTT CTC CAT GGT CGT GA-3 (reverse)