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Application of MALDIMSI across diverse malignanciesincluding colorectal, lung, breast, liver, and brain tumorshas uncovered spatially organized metabolic gradients associated with hypoxia, immune infiltration, stromal composition, and therapeutic response, thereby providing mechanistic insights into regional immune suppression and functional divergence of immune cells within the TME (98, 99)

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sedebokerense cells was observed by 3 DPI, which was in accordance with the fluorescence intensity detected by using the flow cytometry (Fig

Animal model All animal experiments were performed under a National Institutes of Health Animal Care and Use Committee (NIHACUC) approved protocol