Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template
Higher glutathione levels influence red blood cell count, hematocrit, and hemoglobin
In humid climates, these folds can become sites of sweating, friction, itching, pigmentation, intertrigo, fungal infections or bacterial irritation
No serious systemic adverse events have been documented in peer-reviewed human studies at therapeutic doses
Oct 2006;298(5):243-251