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The current standard operating procedure by the European Center for the Validation of Alternative Methods (ECVAM) recommends the use of MTT reduction as parameter to make prediction on the skin irritancy of substances using EpiSkin TM test method, while IL-1 endpoint is regarded as a useful adjunct to MTT assay 48

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428 Additionally, the release of H 2 S contributes to the protection of the gastrointestinal mucosa, reducing inflammatory responses and oxidative stres