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doi: 10.1038/s41392-025-02167-1 3 SaitoAIshikawaSYangKSawaAIshizukaK

Plasma membrane phosphatidylinositol 4-phosphate is necessary for virulence of Candida albicans

Plasmid pBV220 (an ampicillin-resistant and inducible expression vector, carrying the PRPL promoter) gshI gene: Forward primer : 5-ATTTACCGcaattg ATGATCCCGGACGTATCAC-3 Reverse primer : 5 -AATTACCGcaattgTCAGGCGTGTTTTTCCAG-3 caattg is Mun site gshII gene: Forward primer : 5 -TTAACCGgtcgacAATTGCCATGACTGCCC-3 Reverse primer : 5 -TTAACCGgtcgacTTACTGCTGCTGTAAACG-3 gtcgac is SalI site gshI b gene : Forward primer : 5 -ACCGAATTCctgcagGCATTCAAAGCAGAAGGC-3 Reverse primer : 5 - ACCGAATTCctgcagTCAGGCGTGTTTTTCCAG-3 ctgcag is PstI site Plasmid PBV01: gshI gene was inserted into pBV220 plasmid treated with EcoRI to generate plasmid pBV01

Long-term moderate drinking (12 drinks, 23 times per week, timed 4+ hours before injection) is compatible with sermorelin therapy and allows patients to retain 7080% of treatment benefits

This helps boost glutathione production