The filtrate was diluted 200 or 1,000 times with 0.1% formic acid in Milli-Q (MQ) water and then applied to an LCMS-8040 system (Shimadzu Co.) to quantify felinine and its related compounds, 3-methylbutanol glutathione (MBG), 3-methylbutanol cysteinyl-glycine (MBCG), and N -acetylfelinine, based on a previous report (Futsuta et al., 2018)
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Because most peoples gut bacteria cannot produce it efficiently, supplementing is the more reliable route
The study demonstrated that the treatment of BPH with cetrorelix was safe and led to long-term improvement 33 (rapid symptomatic improvement of BPH sustained for 6 months)