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Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

The arrA, arsC, and arsM gene copy numbers were somewhat upregulated with Si-RHBC, but this effect did not reach statistical significance

HPA Axis / Biomarkers Cadegiani & Kater (2017) EROS Study Resting cortisol is normal in 75% of OTS studies

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The immune cells release three important cytokines including tumor necrosis factor-alpha (TNF-), interleukin-1 beta (IL-1), and interferon-gamma (IFN-) which control luteal activity throughout different phases (154)