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Histology Tissue specimens from the brain, liver, spleen, stomach, duodenum, lungs and heart were obtained from rats with superior sagittal sinus ligation at 15 min (and 1, 5 and 10 min intervals thereafter), 24 h and 48 h post-ligation

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At these pH values, copper(II) coordination shifts and the complex can partially dissociate, releasing free copper ions