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1c) serves as a control to ensure that equal amount of D2R was present in each sample and that the differences in co-immunoprecipitation of DAT were due to changes in the degree of D2R-DAT interaction, rather than antibody artifacts

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Although these findings are primarily derived from murine models, they highlight the dual nature of the gutmammary axis as both a conduit for pathological signals and a mediator of mucosal immune defense, warranting further investigation in dairy cows