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Nature 558, 7379

Therefore to understand hemolytic anemia associated with G6PD deficiency, we first need to define the physiologic role of G6PD and pinpoint why the red blood cells are deficient in G6PD

Generally, protein purification entails five essential steps, i.e., extraction from biological matter, separation from lipids and nucleic acids (non-protein components), precipitation step, recovery of bulk protein from raw extract, utilization of hydrophobic or ion-exchange chromatography column for the separation of target protein from bulk protein, and more refined steps including affinity matrix to obtain highly refined target protein with high yields

Since then, however, interest has exploded

Ethics statement The studies involving human participants were reviewed and approved by Institutional Review Board at Nova Southeastern University in Fort Lauderdale, FL, USA