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Trindade et al., 2022

The diluent is added slowly against the inside wall of the vial rather than directly onto the powder, and the vial is swirled gently rather than shaken vigorously, because mechanical agitation can stress peptide structure and generate foaming

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The bacteriostatic water should trickle down the inside of the glass wall and pool gradually at the base of the vial, wetting the powder from beneath rather than hitting it from above