The 3-D cell model was made by culturing cells (2 10 5 1.0 10 6 ) with high glucose DMEM supplemented with 30% bovine collagen solution overnight
According to the European Medicines Agency assessment report on HPL, lyophilized 65% ethanol extracts (5:1, 100 mg of extracts were equivalent to 500 mg of HPL) were standardized to 0.3% hypericin and contained 1.09% rutin, 0.65% hyperoside, 0.45% isoquercitin, 0.04% quercitrin, and 0.07% quercetin (the HPL flavonoid content determination is described in Supplementary method I, Supplementary Figure S1, and Supplementary Tables S1, S2)
No habit-forming tendencies were reported for ME 12 Injection
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